Table 1.

Scoring table for tissue sections obtained from Aire^−/− and Aire+/+ HLA-DR2b and HLA-DR4 tg mice.

Score	Average no. of lesions/section (2–3 sections per slide)
0	No lesions at all
1	1–4 lesions
2	5–15 lesions
3	15–25 lesions

The tissue sections were obtained from the Aire^−/− and Aire^+/+ HLA-DR2b and HLA-DR4 tg with 2 slides per mouse (staggered sections ensuring spanning across the organ). The scoring was performed based on the average number of lesions per section with 2–3 sections per slide.

mice were incubated with tissue sections of organs from C57BL/6 SCID mice, followed by staining with FITC goat anti-mouse IgG Ab (Jackson Immunoresearch).