Table 2.
Incidence of lymphocytic infiltrates in the organs of Aire−/− and Aire+/+ HLA-DR2b and HLA-DR4 tg mice.
| CD4+ T cells | CD8+ T cells | |||||||
|---|---|---|---|---|---|---|---|---|
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| Organ | Liver | Pancreas | Liver | Pancreas | ||||
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| Genotype | Male | Female | Male | Female | Male | Female | Male | Female |
| Aire+/+ HLA-DR2b mice | 0/5 | 0/3 | 0/5 | 0/3 | 0/5 | 0/3 | 0/5 | 0/3 |
| Aire−/− HLA- DR2b mice | 1/11 | 3/7 | 1/11 | 0/7 | 0/11 | 1/7 | 0/11 | 0/7 |
| Aire+/+ HLA-DR4 mice | 0/7 | 0/4 | 0/7 | 0/4 | 0/7 | 0/4 | 0/7 | 0/4 |
| Aire−/− HLA- DR4 mice | 0/8 | 3/7 | 0/8 | 1/7 | 0/8 | 1/7 | 0/8 | 1/7 |
Immune infiltration was examined in Aire−/−, Aire+/+ HLA-DR2b and HLA-DR4 tg mice by I.F. staining on tissue sections from different organs (liver, lung, pancreas, stomach and intestine) with anti-CD4 and anti-CD8 mAbs as described in Materials and Methods. Infiltrates were mostly observed in liver and pancreas of a few aged Aire−/− HLA-DR tg mice. Shown are the incidences of mice that have immune infiltrates found in indicated organs (n = 3–11).