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Effectors generated without exposure of CSE or CSE added only from days 2–4 of culture only were analyzed for Th1 cytokine production by intracellular staining with (A) representative staining and (B) analysis of dual cytokine producers from triplicate conditions shown. T-bet levels in effectors was also determined with (C) representative staining and (D) MFI analysis from triplicate wells shown (one of 2 experiments).
