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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Cell Immunol. 2018 Jun 18;331:121–129. doi: 10.1016/j.cellimm.2018.06.005

Figure 6. Exposure to CSE reduces memory potential in Th1-polarized effector cells.

Figure 6

Effectors were generated by stimulation with either APC and peptide or with anti-CD3 and anti-CD28 antibodies. On day 4, live effector cells were isolated from cultures and analyzed for expression of the α chain of the IL-7 receptor (CD127). Representative staining and MFI analysis from triplicate wells is shown for cells exposed or not to CSE from cultures stimulated with APC and peptide (A and B) or by antibody-mediated activation (C and D). Equal numbers of live effectors exposed to CSE were thoroughly washed and re-plated in media alone for 3 additional days to form in-vitro generated memory cells. Shown is the ratio of memory cells recovered from effectors not exposed to CSE versus from effectors exposed to CSE during priming using both methods of CD4 T cell activation (E and F). Results representative from experiments repeated twice for both APC and peptide and antibody-mediated effector generation.