Figure 4.

Less Conservation of Proximal N-Acetylated CysLys Pairs in 52 Other Mammals and Bird Species

(A) Example of pair conservation.

(B) CysLys pairs are less conserved if they are N-acetylated or located in the matrix.

(C) Conservation of cytosolic N-acetylated CysLys pairs is less than other CysLys pairs.

(D) N-acetylated CysLys pairs are less conserved than non-acetylated CysLys pairs in the cytosol. Data are the average conservation of N-acetylated pairs relative to non-acetylated pairs per species.

(E) Cytosolic N-acetylated CysLys pairs are less conserved than non-acetylated CysLys pairs. Data are the conservation of N-acetylated pairs relative to non-acetylated pairs in each species with linear regression lines and 95% confidence intervals. The line at 45° indicates where values should lie if conservation of N-acetylated and non-acetylated pairs was similar.

(F) Cytosolic N-acetylated CysLys pairs are less conserved than SerLys pairs. Data are average conservation of N-acetylated CysLys pair relative to N-acetylated SerLys pairs.

(G) Cytosolic cysteines from N-acetylated CysLys pairs are less conserved than other surface cysteines. Data are the average cysteine, lysine, or serine conservation from cytosolic N-acetylated pairs relative to all other cytosolic surface cysteines, lysines, or serines not part of these N-acetylated pairs.

Data are the mean ± SEM. ^∗∗∗p < 0.001; ^∗∗∗∗p < 0.0001.