Table 2.
Target | Manufacturer | Product ID | Lot no. | Primary dilution | Exposure (min) |
---|---|---|---|---|---|
PGC‐1α | Millipore | AB3242 | 2691399 | 1:1000 | 3 |
LC3‐I/II | Cell Signalling | 4108 | 3 | 1:500 | 2 |
p62 | Sigma | P0067 | 015M4877V | 1:3000 | 3 |
BNIP3 | Dr L. A. Kirshenbaum | Gift | n/a | 1:1000 | 2 |
NIX | Santa Cruz | sc‐166332 | D0114 | 1:200 | 2 |
TFEB | Bethyl | A303‐673A | n/a | 1:4000 | 5 |
LAMP‐1 | Abcam | ab24170 | GR3183900‐1 | 1:1000 | 2 |
LAMP‐2 | Abcam | ab13524 | GR770‐14 | 1:1000 | 3 |
Cathepsin D | Santa Cruz | sc‐6486 | J1111 | 1:1000 | 3 |
Parkin | Cell Signalling | 4211 | 4 | 1:1000 | 2 |
FoxO3 | Cell Signalling | 2497 | 6 | 1:1000 | 2 |
p53 | Dr S. Benchimol | n/a | n/a | 1:50 | 5 |
Beclin 1 | Cell Signalling | 3738 | 3 | 1:1000 | 2 |
Aciculin | (Belkin et al. 1994) | Gift | n/a | 1:2000 | 1 |
Optineurin | Santa Cruz | sc‐166576 | L2915 | 1:1000 | 3 |
VDAC | Abcam | ab14734 | GR243577‐6 | 1:2000 | 1 |