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. 2018 Jul 19;596(16):3505–3529. doi: 10.1113/JP275973

Table 1.

Primary and secondary antibodies employed

Type Abbreviated name Antibody recognising Host Clone Concentration Supplier Catalogue no.
1° Ab α‐DBH Dopamine‐β‐hydroxylase Mouse Monoclonal 1:1000 Millipore MAB308
1° Ab α‐VAChT Vesicular acetylcholine transporter Guinea pig Polyclonal 1:500 Millipore AB1588
1° Ab α‐VIP Vasoactive intestinal peptide Rabbit Polyclonal 1:200–1:400 Novus Biologicals (discontinued) NBP1‐78338
1° Ab α‐nNOS Neuronal nitric oxide synthase Mouse Monoclonal 1:50 Santa Cruz SC‐5302
1° Ab α‐CGRP Calcitonin gene related peptide Rabbit Polyclonal 1:100 Millipore PC205
Type Ab recognising Host Conjugate IF‐colour Concentration Supplier Catalogue no.
2° Ab α‐Mouse Goat AF‐594 Red 1:500

  • Invitrogen

  • Molecular Probes
2° Ab α‐Mouse Goat AF‐488 Green 1:500

  • Invitrogen

  • Molecular Probes
2° Ab α‐Guinea pig Goat DyLight‐488 Green 1:500

  • AbCam

  • ab96959
2° Ab α‐Guinea pig Donkey Biotinylated — 1:500

  • Jackson IR (discontinued)

  • 706‐175‐148
2° Ab α‐Rabbit Goat AF‐594 Red 1:500

  • Invitrogen

  • Molecular Probes
2° Ab α‐Rabbit Goat AF‐488 Green 1:500

  • Invitrogen

  • Molecular Probes
Streptavidin AF‐488 Green 1:500 Invitrogen S32354