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. 2018 Aug 14;8:12085. doi: 10.1038/s41598-018-30157-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Alpha diversity size-based estimation from species richness (A,C,E) and Gini–Simpson index (B,D,F) according to: COI “only metazoa” dataset clustered at 95% similarity level (A,B) 18S “only metazoa” dataset clustered at 97% (C,D), and the combined taxonomically assigned dataset (COI + 18S; E,F). Species richness was estimated asymptotically, by extrapolation to the highest number of reads per site and from the observed values, while Simpson’s diversity was estimated only from asymptotes. Error bars report 95% confidence intervals for extrapolated values. Standard error for asymptotic estimations were estimated by bootstrapping.