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. 2018 Aug 14;8:12123. doi: 10.1038/s41598-018-30640-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generation of epithelial PyMT-1099 murine breast cancer cells. (A) Photomicrographs represent the morphology of epithelial PyMT-1099, NMuMG (E9) and Py2T cells. Magnification, 10X. (B) Genotyping PCR was performed on genomic DNA isolated from PyMT-1099 cells for the presence PyMT oncogene. NMuMG (E9) served as the negative control in addition to a no template control and PyMT primary tumors and Py2T cells served as the positive controls. Uncropped scans from the genotyping PCR (agarose gel) are displayed in Fig. S4.