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. 2018 Aug 14;8:12123. doi: 10.1038/s41598-018-30640-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Long-term TGFβ treatment and cell migration of PyMT-1099 and NMuMG cells. PyMT-1099 and NMuMG (E9) cells were treated for >20 days with TGFβ to create long term (LT) mesenchymal cells. (A) Photomicrographs represent the morphology of untreated parental and LT PyMT-1099 and NMuMG (E9) cells. Magnification, 10X. (B) Graph represents the number of cells migrated per field across the membrane in a Boyden chamber transwell migration assay; n = 3. ****signifies p-value < 0.0001.