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. 2018 Aug 14;8:12088. doi: 10.1038/s41598-018-30623-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Change in the autofluorescence, volume and autofluorescence per volume unit of E. coli cells after 2 hours of ampicillin treatment. E. coli 7705035 cells were treated, with a range of ampicillin concentrations during exponential growth. After 2 hours of treatment, images of 20,000 cells per condition were collected using the ImageStream flow cytometer system. (a) The cell autofluorescence intensity (λ_ex 488 nm/λ_em 530/30 nm). (b) The cell volume. (c) The autofluorescence intensity per volume unit. For each cell, the fluorescence was normalized by the cell volume. Data are represented as a variation relative to a non-treated control. Each value represents the mean (+/−standard error) of the median values of four independent experiments. The asterisk represents significant differences in the ampicillin 1 versus ampicillin 0 (reference) condition according to 1-way ANOVA Dunn’s comparison test p value < 0.05.