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. 2018 Aug 14;8:12088. doi: 10.1038/s41598-018-30623-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of gentamicin and sodium hypochlorite treatment on the autofluorescence of E. coli cells. E. coli 7705035 cells were treated with a range of gentamicin concentrations corresponding to 0 (red), 0.125 (light blue), 0.25 (orange), 0.5 (MIC; light green), 1 (dark green), 2 (brown) and 4 mg/L (violet) or 0.08% sodium hypochlorite (grey) for 3 hours of the exponential growth. Autofluorescence and FSC of treated cells and untreated control were measured after 1, 2 and 3 hours of incubation using a flow cytometer. Cell autofluorescence was normalized by the FSC signal to obtain the autofluorescence per FSC unit. (a), (b) and (c) 1, 2 and 3 hours of gentamicin treatment respectively. The overlap of red, light blue, orange, light green and dark green produces the brown area. (d), (e) and (f) 1, 2 and 3 hours of sodium hypochlorite treatment respectively. This figure presents results of representative experiments, which were independently repeated 4 times.