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. 2018 Aug 14;8:12088. doi: 10.1038/s41598-018-30623-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Autofluorescence as a predictor of the survival of ampicillin-treated E. coli cells. E. coli 7705035 cells were incubated with 4 mg/L ampicillin (MIC). After 2 hours of incubation, the autofluorescence of treated and non-treated control cells was analyzed using the S3e cell sorter. 200 of the least and 200 of the most autofluorescent cells were sorted and plated on LB agar. After overnight growth, colony-forming units (CFU) were counted to assess the bacterial survival. (a) Box plot of the CFUs. (b) The mean ratio (+/−standard error) between the number of CFUs of the most and the least fluorescent cells of treated and non-treated control cells per experiment. Presented data are from six independent experiments. The asterisk represents significant difference according to paired T-test p value: 0.034.