Table 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| V. alginolyticus | ||
| VIO5 | VIK4 (Rifr Pof+ Laf−) | 36 |
| LPN1 | VIO5 ΔflhF (Rifr Pof+ Laf−) | 4 |
| E. coli | ||
| DH5α | Recipient for DNA manipulation | |
| BL21(DE3) | Novagen | |
| BL21(DE3)/pLysS | ||
| Plasmids | ||
| pBAD33 | Cmr, PBAD | 37 |
| pTY57 | Cmr, PBAD, MCS from pBAD24 | 38 |
| pCold IV | Ampr, PcspA | Takara |
| pAK322 | flhF (wt) in pBAD33 | 3 |
| pAK325 | flhF-egfp (wt) in pBAD33 | 4 |
| pTSK110 | flhF-his6 in pCold IV | This study |
| pTSK119 | flhF-his6 in pTY57 | This study |
| pSK102 | flhF (E440K) in pBAD33 | 21 |
| pSK111 | flhF (R334A) in pBAD33 | This study |
| pSK202 | flhF-egfp (E440K) in pBAD33 | 21 |
| pSK211 | flhF-egfp (R334A) in pBAD33 | This study |
| pSK301 | flhF-his6(G299A) in pCold IV | This study |
| pSK302 | flhF-his6(G304A) in pCold IV | This study |
| pSK303 | flhF-his6(K305A) in pCold IV | This study |
| pSK304 | flhF-his6(T306A) in pCold IV | This study |
| pSK305 | flhF-his6(K311A) in pCold IV | This study |
| pSK306 | flhF-his6(D377A) in pCold IV | This study |
| pSK307 | flhF-his6(G380A) in pCold IV | This study |
| pSK308 | flhF-his6(T436M) in pCold IV | This study |
| pSK309 | flhF-his6(K437A) in pCold IV | This study |
| pSK310 | flhF-his6(D439A) in pCold IV | This study |
| pSK311 | flhF-his6(E440K) in pCold IV | This study |
| pSK312 | flhF-his6(D470A) in pCold IV | This study |
| pSK313 | flhF-his6(R334A) in pCold IV | This study |
| pTrc-flhG | his6-tev-flhG in pTrcHisB | 8 |
Ampr, ampicillin-resistant; Cmr, chloramphenicol-resistant; Rifr, Rifampicin-resistant; Pof+, possessing a polar flagellum; Laf−, lack of lateral flagella.