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. 2018 Jun 6;12(9):2114–2128. doi: 10.1038/s41396-018-0156-3

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© International Society for Microbial Ecology 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Characterization of GN PICI replication origins. a Comparative map of the replication origins of several E. coli PICIs. The iterons are represented by arrows, and their sequences are shown at left. Note that there are always two sets of iterons flanking an AT rich region, which could be the melting site. b Testing EcCICFT073 pri-rep-ori function. pK03Blue derivative plasmids pri-ori, pri-∆ori or ∆pri-ori were tested for maintenance in DH5α on selective agar at the permissive (30 °C) or restrictive (44 °C) temperature. A One-way ANOVA with Tukey’s multiple comparisons test was performed to compare mean differences within rows. Adjusted p values were as follows: pri-ori 44 °C vs pri-∆ori 44 °C < 0.0001^****, pri-ori 44 °C vs ∆pri-ori 44 °C < 0.0001^****