Fig. 4.

Functionality of the EcCICFT073 cos sites. EcCICFT073 cat wt, or its derivatives carrying mutations in the cos1 or/and cos2 sites, were introduced in the lysogenic strains for phages λ or ϕ80. The different strains were MC induced (2 μg/ml) and the transfer of the island quantified. A t-test was performed to compare the wt against the different cos mutants. Adjusted p values were as follows: Phage Lambda EcCICFT073 ∆cos1 = 0.002^**, Phage Lambda EcCICFT073∆cos2 = 0.0266^*, Phage 80 EcCICFT073 ∆cos1 = 0.003^***, Phage ϕ80 EcCICFT073 ∆cos2 = 0.0002^***