NOTCH2NLB Increases Human Cortical Progenitor Maintenance through Its EGF Repeats
(A–F) Immunostaining for GFP (green), PAX6 (red), and βIII tubulin (blue) in control ([A], GFP alone) and NOTCH2NLB-expressing human cortical cells (B–D) 7 days post-lentiviral infection at 30 days of differentiation and quantification of proportion of marker expression among GFP-positive cells (E and F).
(G) Structures of NOTCH2NL full-length (FL) and deletion mutants.
(H–O) Immunostaining for GFP (green), PAX6 (red), and βIII tubulin (blue) in control, NOTCH2NL FL and mutants, or NICD expressing cortical cells 7 days following lentiviral infection and quantification of proportion of marker expression among GFP-positive cells (O).
Data are represented as mean ± SEM and p value: one-way ANOVA and Bonferroni correction. Scale bars, 50 μm (A and H) and 10 μm (C, D, M, and N). See also Figure S3.