Figure S4.

NOTCH2NL Activates Notch Signaling in Human Cortical Progenitors In Vitro, Related to Figure 6

(A–E) Notch signaling activity was examined by HES1 immunoreactivity as a positive readout of NOTCH signaling in human cortical cells, 3 days following lentiviral infection with control vector (GFP only) or leading to overexpression of NOTCH2NL full length (N2NL-FL), NOTCH2NL-EGF repeats deletion (ΔEGF) or NICD.

(F) Quantification of HES1-immunoreactive cells among GFP labeled cells in each condition.

Data are represented as mean ± sem and p values by one-way ANOVA and bonferroni post hoc test. Scale bars; 100μm (A) and 20μm (B).