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. 2018 Aug 15;7(8):61. doi: 10.1038/s41389-018-0074-2

Fig. 5. SOX2 upregulates PIK3CA expression in nasopharyngeal carcinoma to activate PI3K/AKT signaling.

Fig. 5

a Effects of SOX2 knockdown on PIK3CA protein expression in HNE-1 and C666-1 cells. b qRT-PCR analysis of effects of SOX2 knockdown on PIK3CA mRNA expression. c Schematic diagram of putative SOX2-binding sites in PIK3CA promoter. d ChIP assays on two SOX2-binding sites of PIK3CA promoter were performed in CNE2 and HNE-1 cells. e Analysis of the relative luciferase activity in HNE-1 cells cotransfected with wild type or the various mutant PIK3CA promoters. f Overexpression of PIK3CA rescues SOX2 knockdown-inhibited AKT activation. g Overexpression of PIK3CA rescues SOX2 knockdown-inhibited cell proliferation. h Overexpression of PIK3CA rescues SOX2 knockdown-inhibited cell colony formation. i Correlation of expression of PIK3CA with SOX2 in head and neck patients from a TCGA data sets. Error bars ± SD. *P < 0.05. Data are representative from two independent experiments