Fig. 2.

GCL knock down in neurons in vivo causes oxidation of important proteins in the hippocampus. (A) Analysis of carbonylated proteins in different brain areas. Proteins obtained from different brain areas of the 9 months-old CK2a-Cre/+ and CK2a-Cre/shGCL^SFL male mice were solubilized and carbonyl groups were derivatized with Bodipy-Hydrazide. Protein samples (10 μg) were separated in monodimensional polyacrylamide gel electrophoresis (PAGE). For total protein detection, gels were stained with Flamingo fluorescent stain. (B) Two dimensional PAGE (2D-PAGE) (pH range 3–10) of Bodipy-derivatized proteins and Flamingo staining. Arrows indicate spots corresponding to the most carbonylated proteins. Representative images are shown. Cb, cerebellum; Str, striatum, Hip, hippocampus; PCx, prefrontal cortex.