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. 2018 Aug 8;9:1840. doi: 10.3389/fimmu.2018.01840

Table 2.

DNA sequence encoding protease cleavage sites used for plasmid cloning.

Cleavage site
s: sense
as: antisense
Oligonucleotide, 5′ → 3′
KVPLAA (s) CCGGTGGCGGCAAGGTTCCACTTGCCGCCGGAGGAGGC
KVPLAA (as) GGCCGCCTCCTCCGGCGGCAAGTGGAACCTTGCCGCCA
IEPDG (s) CCGGTGGCGGCATTGAACCAGATGGTGGTGGAGGAGGC
IEPDG (as) GGCCGCCTCCTCCACCACCATCTGGTTCAATGCCGCCA
IGNRS (s) CCGGTGGCGGCATTGGTAACAGATCAGGTGGAGGAGGC
IGNRS (as) GGCCGCCTCCTCCACCTGATCTGTTACCAATGCCGCCA
PTSYG (s) CCGGTGGCGGCCCAACATCATATGGTGGTGGAGGAGGC
PTSYG (as) GGCCGCCTCCTCCACCACCATATGATGTTGGGCCGCCA
YRFKG (s) CCGGTGGCGGCTATCGCTTCAAGGGTGGTGGAGGAGGC
YRFKG (as) GGCCGCCTCCTCCACCACCCTTGAAGCGATAGCCGCCA
DVAHKQL (s) CCGGTGGCGGCGATGTTGCCCACAAGCAGCTTGGAGGAGGC
DVAHKQL (as) GGCCGCCTCCTCCAAGCTGCTTGTGGGCAACATCGCCGCCA
VGPDFGRG (s) GTACAAGGGCGTAGGTGGCTCCGGTGGCGGAGTGGGACCCGACTTCGGAAGGGGCGGAGGAGGC
VGPDFGRG (as) GGCCGCCTCCTCCGCCCCTTCCGAAGTCGGGTCCCACTCCGCCACCGGAGCCACCTACGCCCTT
RIEADS (s) GTACAAGGGCGTAGGTGGCTCCGGTGGCGGAAGAATTGAAGCAGACTCAGGCGGAGGAGGC
RIEADS (as) GGCCGCCTCCTCCGCCTGAGTCTGCTTCAATTCTTCCGCCACCGGAGCCACCTACGCCCTT
shRNA (CD95) GATCCAGCGTATGACACATTGATTCTCAAGAGAAATCAATGTGTCATACGCTTCTTTTTTGGAAA
shRNA (scrambled) GATCCAATCTCATTCGATGCATACTTCAAGAGAGTATGCATCGAATGAGATTCCTTTTTTGGAAA