Table 1. FAP expression profiling in various tumor types.
Recent studies on patterns of FAP expression are summarized by tissue of cancer origin, cell type observed to express FAP, and methods of detection used. The relevant reagents, mostly antibodies, are reported with the same level of detail available in each study’s material and methods section.
| Cancer | Cell | Method | Reagents | Reference |
|---|---|---|---|---|
| Bone | Tumor | IHC | Mouse antibody (Santa Cruz Biotechnology, Inc.) | 98 |
| Brain | Astrocyte, stem cell, stroma, macs/fibrocyte | ELISA, WB, IF, qRT-PCR | DuoSet ELISA kit (R&D systems), rat monoclonal D8 and D28 (Vitatex), mouse monoclonal F19 | 22 |
| Breast | Fibroblast | IHC, IF, qRT-PCR | NR | 16 |
| Breast | Stroma, tumor | IHC | Rabbit polyclonal antibody ab53066 (Abcam) | 34 |
| Breast | Stroma, tumor | IHC | Polyclonal antibody (Abcam) | 36 |
| Breast | Stroma, tumor | IHC | Polyclonal antibody (Abcam) | 35 |
| Breast | NR | qRT-PCR | 99 | |
| Colorectal | Stroma | IHC | Unspecified antibody (Abcam) | 47 |
| Esophageal | NR | WB, RT-PCR | Rabbit polyclonal antibody N1N3 (GeneTex, Inc.) | 19 |
| Esophageal | Stroma | ELISA, IHC, WB, qRT-PCR | DuoSet ELISA kit (R&D systems), sheep polyclonal antibody AF3715 (R&D) | 25 |
| Esophageal | Stroma | IHC | Unspecified antibody (Abcam) | 54 |
| Gastric | Stroma | IHC, IF | Unspecified antibody (Boster Biological Technology) | 48 |
| Gastric | Mesothelia | IHC | Rabbit polyclonal antibody (Abcam) | 40 |
| Liver | Stroma | IHC | Rabbit polyclonal antibody SAB2900181 (Sigma) | 59 |
| Liver | Stroma | IHC | Rat monoclonal D8 (Vitatex) | 53 |
| Lung | Tumor | IHC | Rabbit polyclonal antibody (Assay Biotech) | 31 |
| Oral | Tumor | IHC, IF | Rabbit polyclonal antibody (LifeSpan BioSciences) | 51 |
| Ovarian | Stroma, tumor | IHC | Rabbit polyclonal antibody LS-A8023 (LifeSpan BioSciences) | 52 |
| Ovarian | Tumor, mesothelia | IHC, ISH | Rabbit polyclonal antibody ab53066 (Abcam) | 39 |
| Pancreatic | Stroma, tumor | ELISA, IHC, IF | DuoSet ELISA kit (R&D systems), polyclonal antibody (LSBio), mouse monoclonal F19 | 20 |
| Pancreatic | Stroma, tumor | IF | Mouse monoclonal F11-24 (Santa Cruz Biotechnology, Inc.) | 21 |
| Pancreatic | Stroma, tumor | IHC | Rabbit polyclonal antibody ab53066 (Abcam) | 37 |
| Pancreatic | Stroma, tumor | IHC, IF | Sheep polyclonal antibody AF3715 (R7D Systems), mouse monoclonal F19 | 38 |
| Parathyroid | NR | RT-PCR, qRT-PCR | 15 | |
| Renal | Stroma | IHC | Rabbit polyclonal antibody ab53066 (Abcam) | 45 |
| Renal | Stroma | IHC | Rabbit polyclonal antibody ab53066 (Abcam) | 46 |
ELISA: enzyme linked immunosorbent assay. IF: immunofluorescence. IHC: immunohistochemistry. ISH: in situ hybridization. NR: not reported. (q)RT-PCR: (quantitative) reverse transcriptase polymerase chain reaction. WB: western blot.