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. 2018 Aug 8;9:881. doi: 10.3389/fphar.2018.00881

FIGURE 2.

FIGURE 2

Lycorine promotes CRC apoptosis via caspase-dependent pathway. (A,B) RKO and SW480 cells were treated with indicated concentrations of lycorine for 48 h. The protein levels of Cleaved caspase3 and PARP were detected by Western blot assays. (C,D) Activity of caspase-3 was evaluated by caspase colorimetric assays. (E–H) RKO and SW480 cells were treated with lycorine with and without Z-VAD-FMK respectively. (E,F) The levels of cleaved caspase-3 and PARP proteins were determined by Western blot assays. (G,H) Cell viability was measured by MTT assay. For (C,D), data are shown as mean ± SD (n = 3); ∗P < 0.05; ∗∗P < 0.01 compared with control (Student’s t-test). For (G,H), data are shown as mean ± SD (n = 3); ∗∗P < 0.01 compared with control; ##P < 0.01 compared with control cells treated with lycorine (Student’s t-test). All the western data shown are representative of at least three independent experiments.