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. 2018 Jun 27;37(16):e98724. doi: 10.15252/embj.201798724

Figure 1. Cdc48 regulates cell cycle entry and the critical size in budding yeast.

Figure 1

  1. Ssa1‐Ydj1 chaperones (CHPs) are important for nuclear accumulation of G1‐cyclin Cln3 but also for its proteasomal destruction. However, how these opposing fates are regulated is unknown.
  2. Venn diagram of physical interactors of Ssa1, Hsc82, and Cdc48 that display genetic or physical interactions with Cln3.
  3. Budding frequencies of wild‐type (wt), cdc48‐3, cdc48‐6, and cdc48‐AID newborn daughter cells during growth at the restrictive temperature (37°C) in the presence of auxin to induce degradation of Cdc48‐AID.
  4. Individual volumes at budding of cells as in (C). Mean values (N > 300, thick lines), confidence limits (α = 0.05, thin lines) for the mean and P‐values obtained from t‐tests are also shown.
  5. Individual volumes at budding of wild‐type (wt) and cln3 cells transformed with a centromeric vector empty (ctrl) or carrying the CDC48, UFD1, and NPL4 genes. Mean values (N > 200), confidence limits (α = 0.05, thin lines) for the mean and P‐values obtained from t‐tests are also shown.