Table 1.
Strains used and genotypes
| Strain | Origin |
|---|---|
| CML128 (MATa leu2‐3,112 ura3‐52 trp1‐1 his4‐1 can r) | Gallego et al (1997) |
| CML133 (tTA::LEU2) | Gallego et al (1997) |
| CML203 (CLN3‐3HA::GEN) | Gallego et al (1997) |
| CML211 (cln3::LEU2) | Gallego et al (1997) |
| CML344 (tTA::LEU2 cdc34‐2) | Gallego et al (1997) |
| MAG220 (GAL4‐ER‐VP16::URA3) | This study |
| MAG642 (GAL4‐ER‐VP16::URA3 ydj1::NAT) | This study |
| MAG643 (GAL4‐ER‐VP16::URA3 ydj1::NAT CLN3‐3HA::GEN) | This study |
| MAG925 (CLN3‐3HA::GEN GAL1 p ‐CDC48::NAT) | This study |
| MAG927 (GAL1 p ‐CDC48::NAT) | This study |
| MAG1279 (NPL4‐3HA::GEN) | This study |
| MAG1282 (OTU1‐3HA::GEN) | This study |
| MAG1283 (BRE5‐3HA::GEN) | This study |
| MAG1285 (RPN11‐3HA::GEN) | This study |
| MAG1470 (CLN3‐3HA::GEN CDC48‐AID::NAT OsTIR1::URA3) | This study |
| MAG2021 (ydj1::HPH CLN3‐3HA::GEN CDC48‐AID::NAT OsTIR1::URA3) | This study |
| BY4742 (MATα leu2Δ0 ura3Δ0 his3Δ0 lys2Δ0) | Brachmann et al (1998) |
| cdc48‐3 (cdc48‐3 in BY4742) | Tran et al (2011) |
| MAG201 (cdc48‐3 CLN3‐3HA in BY4742) | This study |
| MAG202 (CLN3‐3HA in BY4742) | This study |
| MAG1980 (far1::HPH cdc48‐3) | This study |
| MAG1981 (far1::HPH) | This study |
| DBY2064 (MATα lys2‐801 his4‐619) | D. Botstein |
| KFY205 (cdc48‐6ts in DBY2064) | K‐U. Fröhlich |
| BF305 (GAL1 p ‐CLN3::URA3 cln1::HIS3 cln2::TRP1) | B. Futcher |