Table 2.
Plasmids used and relevant genetic details
| Plasmid | Origin |
|---|---|
| pCM194 (ARS‐CEN URA3 CLN3‐3HA) | Gallego et al (1997) |
| pCM357 (ARS‐CEN URA3 tet p ‐GFP) | Gallego et al (1997) |
| pCYC41 (ARS‐CEN URA3 CLN3‐1‐3HA) | Wang et al (2004) |
| pCYC354 (ARS‐CEN URA3 CLN3ΔHD‐3HA) | Vergés et al (2007) |
| pCYC418 (ARS‐CEN URA3 tet p ‐GFP‐CLN3HD) | Vergés et al (2007) |
| pMAG149 (ARS‐CEN TRP1 CDC48 UFD1 NPL4) | This study |
| pMAG526 (ARS‐CEN TRP1 GAL1 p ‐CDC48) | This study |
| pMAG862 (ARS‐CEN URA3 CLN3‐3HA‐UBI K11R,K48R) | This study |
| pMAG864 (ARS‐CEN URA3 UBI K11R,K48R ‐CLN3‐3HA) | This study |
| pMAG1217 (ARS‐CEN URA3 CLN3 allKR ‐3HA) K(1–580)R substitutions | This study |
| pMAG1219 (ARS‐CEN URA3 CLN3 NtKR ‐3HA) K(1–400)R substitutions | This study |
| pMAG1221 (ARS‐CEN URA3 CLN3 CtKR ‐3HA) K(401–580)R substitutions | This study |
| pMAG934 (ARS‐CEN TRP1 GAL1 p ‐CDC48‐6FLAG) | This study |
| pMAG962 (ARS‐CEN TRP1 GAL1 p ‐CDC48 AA ‐6FLAG) | This study |
| pMAG966 (ARS‐CEN TRP1 GAL1 p ‐CDC48 EE ‐6FLAG) | This study |
| pMAG1027 (ARS‐CEN LEU2 CDC48) | This study |
| pMAG1028 (ARS‐CEN LEU2 CDC48 AA) | This study |
| pMAG1030 (ARS‐CEN LEU2 CDC48 EE) | This study |
| pMAG1388 (ARS‐CEN LEU2 CDC48 Y834A) | This study |
| pMAG1389 (ARS‐CEN LEU2 CDC48 Y834E) | This study |
| pMAG1505 (ARS‐CEN LEU2 CDC48‐6FLAG) | This study |
| pMAG1507 (ARS‐CEN LEU2 CDC48 AA ‐6FLAG) | This study |
| pMAG1663 (2 μm URA3 GAL1 p ‐GST‐CDC48) | This study |
| pMAG1665 (2 μm URA3 GAL1 p ‐GST‐CDC48 AA) | This study |
| pMAG1023 (ColE1 bla lacI q tac p ‐GST‐CDC48 500–683) | This study |
| pMAG1025 (ColE1 bla lacI q tac p ‐GST‐CDC48 500–683AA) | This study |
| pMAG665 (ColE1 bla NEO CMV p ‐HsCDC48) | This study |
| pMAG938 (ColE1 bla NEO CMV p ‐HsCDC48 AA) | This study |
| pMAG940 (ColE1 bla NEO CMV p ‐HsCDC48 EE) | This study |