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. 2018 Jul 26;37(16):e99429. doi: 10.15252/embj.201899429

Figure EV3. Effects of CRAF‐MAPK inhibitors on the expression of Th17‐associated factors.

Figure EV3

  1. Th17 cells were treated with U0126 5 μM or sorafenib 10 μM, and the intracellular protein levels of IL‐17, RORγt and FOXP3 were monitored by FACS analyses. Shown are data from two independent experiments.
  2. Naïve T cells were differentiated over 6 days into Th1 cells. The cells were treated for the last 3 days with 20 μg/ml Vi polysaccharide and CD4+ IFN‐γ+ levels were compared to PBS‐treated cells. Data shown are from three independent experiments.
  3. The murine cells were initially supplemented with 40 ng/ml IL‐23 (high); 20 ng/ml IL‐23 (normal) or 10 ng/ml IL‐23 (low) and then routinely supplemented with either 20 ng/ml; 10 ng/ml or 5 ng/ml IL‐23 during the culture. The surface PHB expression was analysed via flow cytometry. Shown here are data from five independent experiments. Significance levels were calculated by using Bayesian statistics. Statistical significance levels are annotated as NS = not significant P > 0.05.
Data Information: In (B and C), the error bars represent ± SEM.Source data are available online for this figure.