Figure 1. Working model of the GET pathway in yeast.

Newly synthesized TAs are captured by Sgt2 via an unknown mechanism (“?” mark) after their release from the ribosome. TAs are transferred from Sgt2 to the ATPase Get3 with the help of the scaffolding Get4/5 complex. Get3 delivers TAs to the ER membrane by interacting with the Get1/2 receptor complex. Sgt2 contains three functional domains: the N‐terminal domain (NTD) that mediates its homodimerization, the tetratricopeptide repeat (TPR) domain that interacts with heat‐shock proteins, and the C‐terminal domain (CTD) that contains the TA‐binding site.