Figure EV3. cpSRP43 can serve as a trap for TA proteins.

1. cpSRP43 prevents Bos1 aggregation in a dose‐dependent manner, as analyzed by the turbidity assay.
2. The intrinsic dissociation rate constants of Bos1 from the Sgt2‐WT·Bos1 and Sgt2‐TPRmt·Bos1 complexes reconstituted via IVT in E. coli extract. Bos1^CM was translated in the E. coli S30 translation extract coupled to amber suppression, which co‐translationally incorporates the non‐natural fluorescent amino acid L‐(7‐hydroxycoumarin‐4‐yl)ethylglycine (CM) into Bos1 near the TMD, in the presence of 2 μM His₆‐Sgt2‐WT^BFL or His₆‐Sgt2‐TPRmt^BFL, as described in (Rao et al, 2016). The resulting His₆‐Sgt2‐WT^BFL·Bos1^CM or His₆‐Sgt2‐TPRmt^BFL·Bos1^CM complexes were purified using metal affinity resin (Rao et al, 2016). Bos1^CM release from Sgt2‐WT^BFL or Sgt2‐TPRmt^BFL was measured as outlined in Fig 4E. Values are mean ± SEM, with n = 2.