Time courses of TA transfer from Sgt2 to Get3, measured using the FRET assay in (C), in the presence and absence of 3 μM Ssa1. The transfer reaction was initiated by addition of a mixture of 500 nM Get3
BFL, 500 nM Get4/5, and 2 mM ATP to 200 nM preformed Sgt2·Bos1
CM, and donor fluorescence was monitored over time. The grey lines are fits of the data to equation
(4).