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. 2018 Aug 8;9:887. doi: 10.3389/fphar.2018.00887

FIGURE 5.

FIGURE 5

Effects of SFN treatment before SNI surgery on the results of MWT and SPT. (A) The schedule of this study. SNI was performed on day 0 after acclimation. SFN at a single dose of 30 mg/kg was administered 30 min before SNI surgery. MWT was measured on day 5 and 12 after SNI, respectively. SPT was performed on day 7 and 14 after SNI, respectively. (B) Dendrogram of hierarchical clustering analysis. A total of 16 SNI rats were divided into anhedonia-susceptible and anhedonia-resilient groups by SPT results. Eight of 16 SNI rats were anhedonia susceptible phenotype. (C) Dendrogram of hierarchical clustering analysis. A total of 16 SNI rats were divided into anhedonia-susceptible and anhedonia-resilient groups by SPT results. Five of 16 SNI rats were anhedonia-susceptible phenotype. (D) Ratio of anhedonia-susceptible rats to total SNI rats. The ratio of anhedonia-susceptible rats in vehicle and SFN groups were 50 and 31%, respectively. (E) MWT [Time: F(1,16) = 8.851, P = 0.0089; SFN: F(3,16) = 30.58, P < 0.001; Interaction: F(3,16) = 0.9991, P = 0.4186]. Data are shown as mean ± SEM (n = 5). ∗P < 0.05, ∗∗P < 0.01 or ∗∗P < 0.001. (D) SPT [Time: F(1,16) = 32.05, P < 0.001; SFN: F(3,16) = 361.7, P < 0.001; Interaction: F(3,16) = 7.569, P = 0.0023]. Data are shown as mean ± SEM (n = 5). MWT, mechanical withdrawal test; N.S., not significant; SFN, sulforaphane; SNI, spared nerve injury; SPT, sucrose preference test.