Skip to main content
. 2018 Jul 19;11(2):317–324. doi: 10.1016/j.stemcr.2018.06.013

Figure 2.

Figure 2

COPS2 Interacts with CDK1 and OCT4

(A) Colloidal blue-stained gel of coIP samples. Cell extracts of ESCs overexpressing empty vector (EV), COPS2-FLAG, or COPS5-FLAG were subjected to coIP using anti-FLAG M2 beads. CoIP samples were resolved in an SDS-PAGE gel followed by colloidal blue staining. Blue arrows mark the bands of COPS2-FLAG and COPS5-FLAG. Red triangles and asterisks highlight distinct and identical bands present in COPS2-FLAG and COPS5-FLAG coIP samples, respectively.

(B) Venn diagram of COPS2 and COPS5 interacting proteins identified by coIP and mass spectrometric analysis. COPS2-1 and COPS2-2 are two lists of COPS2-interacting proteins identified in two independent experiments. Detailed information about these proteins is provided in Tables S1 and S2.

(C) CoIP experiments to validate the binding of COPS2 to CDK1 and OCT4. CoIP experiments were performed as described in (A). The resulting coIP samples were subjected to western blot.

(D) CoIP experiments to detect the binding of COPS2 to Cyclins. CoIP experiments were performed as described in (A). The resulting coIP samples were subjected to western blot.