Figure 3.

COPS2 Antagonizes with OCT4 to Promote the G2/M Transition

(A) In vitro CDK1 kinase assays using HeLa cell extract, with or without 300 nM CYCLIN B, 1,800 nM OCT4, and/or 1,800 nM COPS2. Data are shown as mean ± SD of three independent replicates.

(B) HeLa cells were transfected with plasmids overexpressing OCT4 or/and COPS2. Forty-eight hours after transfection, the cells were stained with PI and analyzed by flow cytometry.

(C) Quantification of the percentage of G2/M cells from three independent experiments. Data are shown as mean ± SD of three independent replicates.

^∗∗∗p < 0.001, ^∗∗p < 0.01, ^∗p < 0.05.