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. 2018 Jul 19;11(2):306–316. doi: 10.1016/j.stemcr.2018.06.015

Figure 3.

Figure 3

Hif1a Is Positively Correlated with Tgfbs and Biliary Markers and Negatively Correlated with Hepatocyte Markers in Mouse Liver Development

(A) Microarray gene expression analysis of mouse fetal liver from E9.5 to 17.5.

(B) Immunofluorescence staining for HIF1A (red), HIF2A (red), DLK1 (green), and nuclei (blue, DAPI) in E10.5 mouse liver. Scale bar, 100 μm (upper) or 20 μm (lower).

(C) Correlation analysis of hypoxia- (Hif1a), hepatocyte- (Alb and Rbp4), and cholangiocyte- (others) associated markers in mouse livers from E9.5 to 8-week-old mice.

(D) hiPSC-LBs cultured for 10 days (green: eGFP-iPSC-DE cells [AAVS1:EGFP]; red: KO1-HUVECs [MSCV-KO1]; no label: MSCs; scale bar, 250 μm).

(E) ELISA on protein secretion in hiPSC-LBs cultured for 10 days (mean ± SD; n = 15 independent experiments; ∗∗p < 0.01 versus Excess-hypoxia, §§p < 0.01 versus Extreme-hypoxia).

(F) Gene expression in hiPSC-LBs cultured for 10 days (mean ± SD; n = 12 independent experiments; ∗p < 0.05 and ∗∗p < 0.01 versus Excess-hypoxia, §§p < 0.01 versus Extreme-hypoxia).