Table 1.
Primers for RT-qPCR amplification of miRNAs
| ID | Primer | Sequence (5′-3′) |
|---|---|---|
| miR-7 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACCAACAAA |
| Forward | GTGGAAGACTAGTGATTTTG | |
| Reverse | TGCGTGTCGTGGAGTC | |
| novel_mir16 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTCTGAAC |
| Forward | GCACCGAAGCTTAGGGTT | |
| Reverse | TGCGTGTCGTGGAGTC | |
| novel_mir18 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACAGAATAC |
| Forward | GCCTATAATGGCTATTGGTA | |
| Reverse | TGCGTGTCGTGGAGTC | |
| novel_mir19 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACGCATCT |
| Forward | ATCCTTGGACCACAGCAG | |
| Reverse | TGCGTGTCGTGGAGTC | |
| novel_mir22 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACACACCT |
| Forward | TGAGGGTGACTGGCAGG | |
| Reverse | TGCGTGTCGTGGAGTC | |
| novel_mir24 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTTCGGC |
| Forward | CACCACTCTTGTCTCTGC | |
| Reverse | TGCGTGTCGTGGAGTC | |
| novel_mir26 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTCGAGT |
| Forward | ATGATGGCAGCGGTGACT | |
| Reverse | TGCGTGTCGTGGAGTC | |
| novel_mir31 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACACTGAC |
| Forward | CTAATTTGAGCCATCTGTCA | |
| Reverse | TGCGTGTCGTGGAGTC | |
| novel_mir34 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACCAAGATG |
| Forward | CACAGCCGTGTAGTCATC | |
| Reverse | TGCGTGTCGTGGAGTC | |
| novel_mir35 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTGGCCA |
| Forward | GTATTGGGCGTGTGTTGG | |
| Reverse | TGCGTGTCGTGGAGTC | |
| .novel_mir37 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTGAGGA |
| Forward | TCGCAGATCCAGAATGTTC | |
| Reverse | TGCGTGTCGTGGAGTC | |
| novel_mir40 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACACTGAAG |
| Forward | TGGAATGCATGGCTACACT | |
| Reverse | TGCGTGTCGTGGAGTC | |
| novel_mir45 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTGTCCG |
| Forward | GGCGTGGCAGGGGTTTC | |
| Reverse | TGCGTGTCGTGGAGTC | |
| novel_mir47 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTCGAGT |
| Forward | ATGATGGCAGCGGTGACT | |
| Reverse | TGCGTGTCGTGGAGTC | |
| novel_mir48 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACACAGGC |
| Forward | TACCCTGATATTCCTTGCC | |
| Reverse | TGCGTGTCGTGGAGTC | |
| novel_mir5 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTTTGGGA |
| Forward | TCAATGCCCTTGGAAATCC | |
| Reverse | TGCGTGTCGTGGAGTC | |
| novel_mir53 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACCTTAGG |
| Forward | GGGTTAGTCGGGTCCTA | |
| Reverse | TGCGTGTCGTGGAGTC | |
| novel_mir7 | RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTCTCACA |
| Forward | GATGACTACACGGCTGTG | |
| Reverse | TGCGTGTCGTGGAGTC | |
| 18S rRNA | Forward | GGAATTCCCAGTAAGCGCAA |
| Reverse | CCAGTCCGAAGGCCTCACTA |
18S rRNA: a reference gene of the S. paramamosain