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Microscope images showing that Pluronic F-127 prevents HUVEC adhesion to the region without fibronectin coating. No HUVEC were attached to the part of the well surface that had not been pre-treated with fibronectin, prior to passivation with Pluronic F-127, after 24 h (a) and 72 h (c) of growth. Without Pluronic F-127 passivation, HUVEC were attached to the surface without fibronectin 24 h after seeding (b) and had proliferated further by 72 h (d). (Scale bar = 500 μm)
