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. 2018 Aug 14;18:402. doi: 10.1186/s12879-018-3315-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — a. Superose 6 column(upper) and Q-Sepharose column (lower) purified F1 protein. P1 and P2 fractions were subsequently pooled together as immunized antigen. b: SDS-PAGE of E. coli-derived recombinant F1 protein purified from Q-Sepharose column. c: Western blot assay of E. coli-derived recombinant F1 probed with anti-F1 mouse serum. 1st Ab: Anti-F1 Mab (YPF19); 2nd Ab: AP-conjugated anti-mouse IgG