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. 2018 Jul 26;11(2):440–453. doi: 10.1016/j.stemcr.2018.06.021

Figure 2.

Figure 2

Effect of Different BMPs on the Forskolin Suppression of Hypertrophic Chondrocyte Gene Expression and Forskolin Enhancement of Sulfated Glycosaminoglycan Production

(A) Effect of replacement of BMP4 in PTB (PDGF + TGF-β+BMP4) with GDF5 (PTG) or BMP7 (PT7) on gene expression during chondrogenesis with (brown) or without (blue) forskolin (Fk). Mean relative expression levels from n = 3–5 (COL2A1), 3–6 (SOX9 and COL10A1), and 3 (RUNX2) with SEM and p values. Additional data are provided in Figure S3A.

(B) Fold changes in expression by Fk addition. Mean values from n = 3–12 shown with SEM and p values. Table S7 shows all n and p values.

(C) Quantitative comparison of the capacity of chondrocytes generated under PTB, PTG, and PT7 with (brown) or without (blue) Fk to produce sulfated glycosaminoglycan (sGAG). Top: Mean values of μg sGAG/μg DNA (n = 4–5) shown with SEM and p values. Bottom: Mean fold changes in the μg sGAG/μg DNA values by Fk treatment with SEM and p values (n = 4).