Figure 5.

Comparative Transcriptome Analysis of Cartilage Pellets Using the RNA-seq Technology

(A) Principal component analysis of the expression pattern of protein-coding genes between PTB (PDGF + TGF-β + BMP4, red) and PTBFk (PTB + forskolin, blue) cartilage pellets.

(B) Heatmap of the differentially expressed genes (DEG).

(C and D) Gene ontology (GO) analysis. GO categories for forskolin (Fk)-induced genes (brown bars) and Fk-suppressive genes (blue bars). (C) Top GO categories (p < 1.0E−17) from Table S1. (D) Skeletogenesis-related GO terms (p < 1.0E−04) from Table S2.

(E and F) Selected lists of Fk-induced genes (E) from Table S3 and Fk-suppressive genes (F) from Table S4.