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. Author manuscript; available in PMC: 2019 Jul 6.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2018 Jul 6;137:63–68. doi: 10.1016/j.prostaglandins.2018.07.001

Figure 3.

Figure 3.

HET0016 and TRPV1 inhibition reduce ROS production. (A, B) TRPV1 knockout (KO), A784168 (A7), and HET0016 each attenuated the ROS production induced by 20-HETE mimetic 20-5,14-HEDGE (HEDGE, A) or 1-h OGD (B), as measured by the presence of 2',7'-dichlorodihydrofluorescein diacetate (DCFDA). The combination of A784168 and HET0016 did not achieve additive effects. (C) Western blot analysis revealed increased carbonyl immunoreactivity, indicative of H-I-induced oxidative stress, in the ipsilateral hemisphere at 3 h of recovery. HET0016 and A784168 each reduced carbonyl immunoreactivity. The combination of A784168 and HET0016 did not achieve additive effects (n=4-7/group). Optical density (OD; mean ± SD) was integrated over multiple protein bands (21–97.4 kDa). *p<0.05 vs wild-type+vehicle (Veh)/control procedure; #p<0.05 vs wild-type OGD/H-I+vehicle.