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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: J Intern Med. 2016 Jun 16;281(1):25–40. doi: 10.1111/joim.12528

Fig. 3.

Fig. 3

Utilization of the deductive ligand–receptor matching strategy for the identification of C-peptide receptor candidates. C-peptide-responsive cell lines were screened for the expression of orphan G protein-coupled receptors (GPCRs). Orphan GPCRs expressed by all responsive cell lines were then knocked down individually using siRNA in KATOIII cells. Cells were treated with C-peptide, and C-peptide signaling was assessed. Knockdown of GPR146 resulted in loss of C-peptide-induced signaling, and thus was considered the best candidate for the C-peptide receptor.