Effects of Notch signaling activation on expression of matrix catabolic and anabolic genes in rAF cells. qPCR analyses of Notch signaling target genes (a), matrix anabolic genes (b), and matrix catabolic genes (c,d) in rAF cells infected with adenoviruses expressing Notch1 intracellular domain (NICD1) (Ad-NICD1) or adenoviruses expressing GFP (Ad-GFP) for 3 days. Relative gene expression was calculated by 2−ΔΔCt method after normalizing first to house-keeping Gapdh, and then to the control groups (Ad-GFP-infected cells). Data are means ± SD. *p <0.05, n = 3