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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: J Cell Physiol. 2018 Jan 19;233(7):5431–5440. doi: 10.1002/jcp.26385

FIGURE 3.

FIGURE 3

Activation of Notch signaling suppressed expression of genes encoding key matrix-degrading enzymes in rNP cells. (a–d) qPCR analyses of Notch signaling target genes (a), matrix anabolic genes (b), and matrix catabolic genes (c,d) in rNP cells infected with Ad-GFP or Ad-NICD1 for 3 days. Relative gene expression was calculated by 2−ΔΔCt method after normalizing first to house-keeping Gapdh, and then to the control groups (Ad-GFP-infected cells). Data are means ± SD. *p <0.05, n = 3. (e) Western blot analysis of Mmp13 in rNP cells infected with Ad-GFP or Ad-NICD1 for 3 days