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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: J Cell Physiol. 2018 Jan 19;233(7):5431–5440. doi: 10.1002/jcp.26385

FIGURE 4.

FIGURE 4

Activation of Notch signaling prevented TNF-α- and inflammatory macrophage-induced mRNA and protein expression of Mmp13 in nucleus pulposus cells. (a–b) qPCR analyses of catabolic Mmp13 in rat nucleus pulposus cells that were infected with Ad-GFP or Ad-NICD1 for 1 day, and then treated with 20 ng/ml TNF-α (a) or 30% conditional medium collected from inflammatory macrophages (M3CM) (b) for 2 days. Relative gene expression was calculated by 2−ΔΔCt method after normalizing first to house-keeping Gapdh, and then to the control groups (Ad-GFP infected cells). Data are means ± SD. *p < 0.05, n = 3. (a–b) Western blot analyses of Mmp13 in rat nucleus pulposus cells. rNP cells were treated as described above