Figure 6.

Leptin induces overexpression of the protein c-ACT and increases phosphorylation of STAT3. (a) The c-ACT protein content was measured by Western blotting of the heart homogenate using GAPDH as the loading control. Bars represent the median ± SEM of four independent mitochondrial preparations. (b) The phosphorylated STAT3 protein content was measured by Western blotting of the heart homogenate using total STAT3 as a reference control. (c) The STAT3 protein content was measured by Western blotting of the heart homogenate using GAPDH as the loading control. The values indicate the mean ± SEM, n = 4; ^∗p < 0.05 versus the control group.