Figure 1.

J-HSPCs Display a Short-Term Repopulating Defect

(A) N- or J-LSK cells (CD45.2) were mixed 1:1 with competitor LSK cells (CD45.1) and injected into lethally irradiated recipient mice (CD45.1/CD45.2). CD45.2⁺ chimerism of the PB was examined by flow cytometry every 4 weeks for 20 weeks post transplant.

(B) Normalized CD45.2⁺ chimerism in total recipient PB after transplant.

(C) Normalized CD45.2⁺ chimerism among the myeloid (GR1⁺CD11B⁺) and lymphoid (B cells as B220⁺ and T cells as CD4⁺CD8⁺) lineages in the recipient PB post transplant.

(D) Normalized CD45.2⁺ chimerism among the BMC at 4, 8, and 12 weeks post transplant. Data presented are normalized to the CD45.2⁺ chimerism observed in recipients of J-LSK cells.

Data from three independent transplants are presented as mean ± SEM. Donor mice, n ≥ 3 pooled per transplant. Recipient mice, n ≥ 5 per transplant. ^∗p < 0.05, ^∗∗p < 0.005, ^∗∗∗p < 0.001, significantly different from recipients of J-LSK cells.