Figure 2.

J and N Mice Have Similar Numbers of Long-Term Functional Repopulating Units

(A) Decreasing numbers of N- or J-BM cells (CD45.2) were mixed with 200,000 competitor BM cells (CD45.1) and injected into lethally irradiated recipient mice (CD45.1/CD45.2). CD45.2⁺ chimerism of the PB was examined by flow cytometry every 4 weeks for 16 weeks post transplant.

(B) CD45.2⁺ chimerism in total recipient PB after transplant.

(C) At 16 weeks post transplant, recipients were scored as non-engrafted or engrafted (i.e., CD45.2⁺ PB chimerism >1% in the T cell, B cell, and myeloid cell lineages) and the frequency of HSC (i.e., CRUs) calculated via Poisson statistics. χ² analysis revealed a good fit to the limiting dilution model.

Data are presented as mean ± SEM. Donor mice, n ≥ 3 pooled per transplant. Recipient mice, n ≥ 7 per transplant.^∗p < 0.05, ^∗∗p < 0.005, ^∗∗∗p < 0.001, significantly different from recipients of J-BM cells.