Figure 3.

J-MMP3 and J-MPP4 Display a Repopulating Defect Relative to N-MPP3 and N-MPP4

(A) Schematic representation of the multipotent progenitor hierarchy and their relative contribution to the PB lineages (adapted from Pietras et al., 2015).

(B) CD45.2 N or J-HSPCs (LT-HSC, ST-HSC, MPP2, MPP3, and MPP4) were transplanted into sublethally irradiated recipients (CD45.1/CD45.2). Recipient PB was examined for CD45.2⁺ cells and myeloid (GR1⁺CD11B⁺) and lymphoid (B cells as B220⁺ and T cells as CD4⁺CD8⁺) cells by flow cytometry every 3–4 days for 33–100 days.

(C) Chimerism percentage in the recipient mice PB after transplant.

Data from two independent transplants are presented as mean ± SEM. Donor mice, n = 3 pooled per transplant. Recipient mice, n = 5 per transplant. ^∗p < 0.05, ^∗∗p < 0.005, ^∗∗∗p < 0.001, significantly different from recipients of J-HSPCs.