Figure 5.

BMC Derived from Transplanted J-HSPCs Display Elevated ROS Levels Post Transplant

N- or J-LSK cells (CD45.2) were mixed 1:1 with competitor LSK cells (CD45.1) and injected into lethally irradiated recipients (CD45.1/CD45.2). Normalized CellROX fluorescence in CD45.2⁺ cells in each BMC after 4 (A), 8 (B), 12 (C), and 20 (D) weeks after transplant. Data presented are normalized to the fluorescence levels in J mice. Data from two independent transplants are presented as mean ± SEM. Donor mice, n ≥ 3 pooled per transplant. Recipient mice, n ≥ 4 per time point. ^∗p < 0.05, ^∗∗p < 0.005, significantly different from recipients of J-LSK cells.