Figure 6.
J-HSPCs Are Hypersensitive to Oxidative Stress Induction Relative to N-HSPCs
(A) Experimental schematic. BMC ROS levels and CFU potential were examined in J and N mice 24 hr after treatment with pI:pC.
(B) CellROX Deep Red fluorescence was measured in BMC from N and J control mice and after pI:pC injection. Fluorescence was normalized to the values of J control cells, and the increase of fluorescence between control and pI:pC injected mice was calculated.
(C–E) Number (C), size (D), and distribution (E) of CFUs generated by HSPCs from N and J control mice and after pI:pC injection.
(F and G) Number (F) and size (G) of pre-B colonies generated by HSPCs from N and J control mice and after pI:pC injection. ZnCl2 was added to CFU and pre-B colonies assays to increase the production of ROS.
Data are presented as mean ± SEM, n ≥ 3. ∗p < 0.05, ∗∗p < 0.005.